| Electrochemical aptasensor based on nanometal /nanocarbon composites as signal amplification modifier for sensitive detection of CEA |
| کد مقاله : 1050-CNF |
| نویسندگان |
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فرزانه حسینی دخت *1، محمد مظلوم اردکانی1، نفیسه صحرائی2 1دانشگاه یزد 2دانشکده شیمی پردیس علوم دانشگاه یزد |
| چکیده مقاله |
| Carcinoembryonic antigen (CEA), as a tumor-associated glycoprotein, has been identified as a broad-spectrum tumor marker to indicate the presence and progression of multiple tumors[1]. Carbon-based nanostructures or nanocomposites have recently attracted substantial attention as one of the best electrode materials because of their outstanding electrical, chemical and mechanical features [2]. Among different noble nanometals, silver nanoparticles (AgNPs) are subjected to new engineering technologies with extraordinarily novel resultant morphologies and characteristics. These NPs have several benefits including simplicity of the preparation process, cost-effective manufacturing method, facilitating more efficient electron transfer (eT), and harboring more active sites on their surface [3]. These advantages make it a suitable choice, and it seems that utilizing AgNPs along with CNT as a specific platform can be a good choice in designing electrochemical sensors and aptasensors. In the current study, a novel and highly selective aptasensor was developed on an electrode surface for the ultrasensitive detection of CEA through an strategy of functionalized GCE with the CNT and AgNPs as an efficient nanocomposite. In the proposed sensing strategy, the amino-aptamer (NH2-Ap) was covalently attached on the Nano metals, which was in turn attached on the surface of a glassy carbon electrode (GCE) modified. By combining the advantages of each nanocomposites component like catalytic activity and stability of CNT, excellent conductivity of AgNPs nanometal,the synthesized nanocomposite exhibited very well electrocatalytic activity in the electrode surface. The electrochemical measurements were investigated using cyclic voltammetry (CV). Furthermore, the electrode response changes at each stage after the modification with the nanomaterials, aptamer and CEA was efficiently investigated using EIS technique. Different concentrations (1.0×10-7-10 ngml-1) of CEA were measured by aptasensor under optimized experimental conditions , and their current responses were recorded by the DPV method. the designed aptasensor showed a wide linear response range of 1.0×10-6-10 ng.mL−1, the low detection limit of 1 fg.mL−1, and good selectivity and excellent detection of CEA in serum samples. Consequently, biosensor based on synthesized nanocomposite represented a collection of very favorable sensing performances, including desirable operating potential, low detection limit, high stability, sensitivity and reproducibility. |
| کلیدواژه ها |
| Electrochemical aptasensor, CNT, Ag nanoparticles, Carcinoembryonic antigen (CEA) |
| وضعیت: چکیده برای ارائه به صورت پوستر پذیرفته شده است |