| Electrochemical paper-based immonuosensor on a ternary nanocomposite for detection of cancer derived exosomes |
| کد مقاله : 1052-CNF |
| نویسندگان: |
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نفیسه صحرائی *1، محمد مظلوم اردکانی1، جواد محیطی اردکانی2، علیرضا مرادی3، علیرضا خوشرو4 1دانشکده شیمی پردیس علوم دانشگاه یزد 2دانشگاه علوم پزشکی شهید صدوقی یزد دانشکده پزشکی گروه بیوشیمی 3دانشگاه علوم پزشکی شهید صدوقی یزد، دانشکده داروسازی ، گروه شیمی دارویی 4دانشگاه علوم پزشکی همدان، مرکز تحقیقات سلامت |
| چکیده مقاله: |
| Exosomes have become a research focus in recent years because of their unique function with cell behavior and disease diagnosis. As nano-sized extracellular vesicles with sizes of 30– 100 nm, exosomes are secreted from various cells in the body, including tumor cells, neurons, immune cells and reticulocytes, and their pathological and physiological significance have been paid attention by researchers. Currently, the exosomes that exist in bodily fluids are thought to carry abundant molecular information derived from their originating cells, including microRNA, carbohydrates and protein, and mediate intercellular communications by conveying information. More importantly, exosomes have been reported to involve in tumor antigens, anti-tumor immune response and tumor diagnostics, which is close related to public health. These unique properties make exosomes become a potential biological candidate for diseases therapy as well as diagnosis. Thus, detection of exosomes provides foundation for further relevant studies. To date, the exosomes should be purified by ultracentrifugation before analysis. Afterwards, exosomes are analyzed by various methods, including western blot, flow cytometry or enzyme linked immunosorbent. Although these methods are effective and robust, the apparent shortcomings such as time-consuming operations, expensive instruments, and rely heavily on the sample handing skills severely limit their widely applications. Hence, the development of a simple strategy to detect exosomes is significant importance, at best as high sensitivity and low cost. Personalized health care (PHC) includes personalized, preventive, predictive and participatory approaches which are of significance in new diagnostics. This personal health care requires fast, accurate and minimally invasive diagnostic tools that make it possible to evaluate and monitor the process of a disease by diagnosing specific disease biomarkers. Point-of-care testing (POCT) involves a wide range of diagnostic tools that meet this purpose. Electrochemical paper-based devices (ePADs) have been introduced as simple, inexpensive, portable and disposable measurement devices to be used in many POCT applications, especially in handling emergencies and outpatient as well as remote usages. Electrochemical detection is a real quantitative detection method with better sensitivity, selectivity and detection limits than indirect measurement methods. In recent years, there has been a revolution in quantitative detections by POCT, thanks to the benefits of electrochemical sensors and paper substrates. In this paper, a cost-effective, biodegradable electrochemical device based on a highly flexible chromatographic paper was fabricated. The electrode modification was performed with materials carbon functionalized with multi-carbon nanotubes and gold nanoparticles. An antibody was immobilized on the porous surface of the materials carbon to combine with proteins overexpressed on the exosomal membrane. This structure effectively protects the electron transfer of a redox prop and leads to quantitative detection of exosomes using current changes. The electrochemical measurements were investigated using cyclic voltammetry (CV), differential palls voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) technique. This paper platform had a high sensitivity (a detection limit of 85 exosomes/µL and a linear range of 5× 102 to 1 × 106 exosomes/µL) and suitable selectivity for detection of exosomes in the real sample. |
| کلیدواژه ها: |
| Paper based electrochemical, biosensor, Exosome, Nanocomposit |
| وضعیت : چکیده برای ارائه به صورت پوستر پذیرفته شده است |