| Nanomolar detection of lansoprazole: Computational assisted to monomer–template-complex study based on molecularly imprinted polymer and electrochemical determination |
| کد مقاله : 1060-CNF |
| نویسندگان: |
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زینب خلیلی *، عزیزاله نژادعلی دانشگاه پیام نور |
| چکیده مقاله: |
| In the present study, a sensitive electrochemical sensor based on a molecularly imprinted polymer (MIP) was developed to measure the lansoprazole (LNS) concentration in real samples. Some electrochemical approaches, including cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques, were used for fabricate the selective MIP–PGE sensor [1]. MIP has been used in applications, including selective extraction, chemical separation, catalysis, and molecule sensing. MIPs are a class of synthetic polymers with particular detection capabilities for template molecules. MIP is a new technique and has several advantages such as low cost, high sensitivity, simplicity, high selectivity and good stability in many solvents and a wide range of laboratory conditions. Template and monomer are the main components in MIP synthesis and can be combined in three ways, covalent, semi–covalent and non–covalent, which is the most commonly used non–covalent imprinting. After the template is extracted at the end of the polymerization process, many cavities are produced that are similar in appearance and size to the template [2, 3]. The most suitable monomer was chosen by a computational method. One of the computational approaches is the density functional theory (DFT) method that is carried out to find the most suitable monomer to bind to the LNS (as a template) by calculating the energy difference (∆E) [3]. An experimental design is a collection of necessary actions to optimize the process variables through statistical modeling. To optimize the critical factors the Plackett–Burman design (PBD) and central composite design (CCD) methods were done [4]. A CV technique was used to synthesize the sensor by electro–polymerization of pyrrole (PY) on a pencil graphite electrode (PGE) in the presence of a template molecule. The DPV technique was performed for all measurements. All measurements were done using a three–electrode cell, consist of MIP–PGE as the working electrode, a platinum auxiliary electrode and an Ag/AgCl reference electrode [3, 4]. The characterization of the modified sensor was investigated by field emission scanning electron microscope. In this research project, linear rang of 10–1000 (µM) and correlation coefficients (R2) of 0.9846 were obtained by plotting the calibration curve under optimal conditions. The limit of detection (LOD) and the limit of quantitation (LOQ) were determined as 0.031 μM (S/N3) and 0.075 μM (S/N10), respectively. Finally, the proposed sensor was successfully applied to LNS determination in capsule and serum samples. This method has also been considered by many researchers due to its ease of use, availability, and low sample consumption. |
| کلیدواژه ها: |
| Imprinted electrochemical sensor; Lansoprazole; Density functional theory; Multivariate optimization |
| وضعیت : چکیده برای ارائه به صورت پوستر پذیرفته شده است |