| Molecularly imprinted polymer-based electrochemical sensor for the determination of L-histidine by using GCE |
| کد مقاله : 1067-CNF (R1) |
| نویسندگان |
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منصوره علیقیان باغ خندان *1، علی بنویدی2، عمادالدین امین صدرآبادی3 1دانشگاه یزد.پردیس علوم پایه.دانشکده شیمی 2شیمی تجزیه، گروه شیمی، پردیس علوم پایه، دانشگاه یزد، یزد، ایران 3گروه تجزیه-دانشکده علوم-دانشگاه یزد-یزد-ایران |
| چکیده مقاله |
| Molecular engraving is the process of creating specific recognition sites in a polymer through a pattern [1]. Unlike complex and time-consuming methods of laboratory-level correction, molecular engraving offers fast, sensitive, inexpensive, easy-to-use, and highly selective approaches to screen, especially for diagnostic applications. Due to their physical and chemical strength, high stability properties, low cost and reusability molecularly based polymer sensors have become very attractive methods for such applications. Molecularly imprinted polymers can be made with different types and combinations of functional monomers, crosslinkers, primers and solvents. The quality and properties of engraved polymers can be changed by combining monomer and crosslink mixtures, laboratory conditions, interaction mechanisms, and so on. Molecularly imprinted polymer has several advantages including easy and cost-effective preparation, high stability, high affinity and selectivity over the pattern molecule [2]. L-histidine is an amino acid. Histidine is used by the body to make certain hormones and metabolites that affect kidney function, nerve conduction, gastric secretions, and the immune system. It is also used to make histamine in the body. This study presented an ultra-sensitive voltametric sensor for the detection of L-histidine based on a glassy carbon electrode (GCE) modified with a thin coating of nanoparticles and electropolymerized molecularly imprinted polymer (MIP). For this purpose, first an electrode with a thin layer of carbon nanofibers (CNF) (CNF/GCE) has been fabricated. The MIP was then synthesized on the surface of the modified electrode through the electro-polymerization of orthophenylenediamine in the presence of L-histidine molecules as matrix polymer and template molecules respectively. During the fabrication process, the L-histidine molecules were embedded in the polymer matrix. Subsequently, the template molecules were removed from the electrode by using alkaline ethanol. The template molecules removal was studied using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscope (SEM). The EIS technique was used for the measurement of the L-histidine in the real sample. The most effective of parameters on the voltametric signal were optimized using response surface methodology. The functional monomer concentration and pH of the polymerization medium were optimized to 10 mM and 5.2, respectively. In the optimized condition, the concentration of L-histidine was determined in serum sample satisfactorily. |
| کلیدواژه ها |
| Electrochemical methods, Nanomaterials, CNF, Molecularly Imprinting Polymer (MIPs) |
| وضعیت: چکیده برای ارائه به صورت پوستر پذیرفته شده است |