| Hematoxylin as an electrochemical label for miR-155 biosensor; selectivity study |
| کد مقاله : 1098-CNF (R2) |
| نویسندگان |
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فاطمه خسروی *1، مصطفی عظیم زاده2 1دانشگاه علوم پزشکی شهید صدوقی (یزد) 2دانشکاه علوم پزشکی شهید صدوقی (یزد) |
| چکیده مقاله |
| In miRNA-based electrochemical biosensors, one of the most important steps is to measure the selectivity and specificity of the biosensor towards its specific target versus other biomolecules available in the sample. Electrochemical labels are one of the popular measurements in the characterization of biosensors. In our fabrication process of miR-155 biosensor, hematoxylin acts as an electrochemical label which had been selected based on a few preliminary experiments. We have used Differential Pulse Voltammetry (DPV) as our major method to evaluate the characteristics of the fabricated electrode. The conventional three electrode system was used with glassy carbon working electrode, platinum counter electrode, and saturated calomel reference electrode. After applying nanomaterials (composite of graphene oxide and gold nanoparticle) and immobilizing complementary DNA sequences on the electrode surface the nanobiosensors was used to measure the miR-155 as our biomarker. Hematoxylin (1 mM solution for 5 minutes) accumulated on oligonucleotides with minor groove interaction type which showed a significantly different peak current based on the miR-155 concentration. Furthermore, one nucleotide mismatch and three nucleotide mismatch. As a label, There was a substantial difference between the presence of hematoxylin and without it; the current without immersing the electrode in its solution was around zero. The peak current of one nucleotide and three nucleotide mismatches was significantly different from the target miRNA. The results showed high sensitivity which brought us to the conclusion that hematoxylin is a great marker in miRNA detection via biosensors. |
| کلیدواژه ها |
| Hematoxylin, Electrochemical label, Biosensor, DPV |
| وضعیت: چکیده برای ارائه به صورت پوستر پذیرفته شده است |